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Image Search Results
Journal: Clinical and Translational Medicine
Article Title: Hepatic interleukin‐1 receptor type 1 signalling regulates insulin sensitivity in the early phases of nonalcoholic fatty liver disease
doi: 10.1002/ctm2.1048
Figure Lengend Snippet: HFD‐induced alterations of MAPK and NF‐κB p65 signalling in the liver of Il1r1 Hep−/– and WT mice. Immunoblotting of (A) phospho‐JNK1/2 (Thr183/Tyr185), (B) phospho‐ERK1/2 (Thr202/Tyr204), (C) phosho‐p38 MAPK (Thr180/Tyr182), (D) phospho‐NF‐κB p65 (Ser536) and total (A) JNK1/2, (B) ERK1/2, (C) p38 MAPK, and (D) NF‐κB p65 protein in liver whole tissue lysates of the different experimental groups after 12 weeks of dietary feeding. In A‐D representative immunoblots with densitometric analysis are shown. * p < .05, *** p < .001 for Il1r1 Hep−/– versus WT using unpaired, two‐tailed Student's t ‐test (A and B). There was no statistically significant difference between Il1r1 Hep−/– and WT mice on HFD with respect of the parameters in C and D
Article Snippet: To mimic steatotic conditions, cells were pretreated with a nontoxic dose of BSA‐Oleate (200 μM, MilliporeSigma) for 18 h. To examine the involvement of specific signalling pathways in mediating IL‐1‐induced effects in murine and human hepatocyte cultures, the
Techniques: Western Blot, Two Tailed Test